English: This work relies on two data sources which, in my opinion, do not rise to the level of copyrightable material. The spectrum is copied from
File:Rendered Spectrum.png by
User:Spigget; hopefully a spectrum is immune from such concerns. The absorption spectrum is taken from an online website of the Oregon Medical Laser Center at
[1], which provides a tabulated set of absorbance measurements for a 10 micromolar solution of methylene blue (path length 1 cm) made by Scott Prahl.
[2] Multiplication of these calculated extinction coefficients (base 10 logarithm) by 0.00001 and 2.303 yields original OD data going up to 1.7 at peak absorption, which should be safely in range of the instrument, an unspecified HP spectrophotometer. The chart was made by me using GIMP from the original dataset as follows. (Because
w:database copyright does not apply in the United States, and so far as I know is not recognized by Commons policy either, I feel free to copy and use this data:)
200 12177
202 11446
204 10687
206 9865
208 9288
210 8810
212 8305
214 7924
216 7698
218 7376
220 7181
222 7114
224 7138
226 7341
228 7684
230 8022
232 8478
234 9146
236 10098
238 11363
240 12888
242 14478
244 15907
246 16885
248 16898
250 15787
252 14101
254 12457
256 11269
258 10573
260 10291
262 10355
264 10811
266 11662
268 12801
270 14127
272 15498
274 16960
276 18695
278 20827
280 23248
282 25937
284 28775
286 31752
288 34813
290 37552
292 39064
294 38802
296 36815
298 33466
300 29334
302 25069
304 21005
306 17413
308 14496
310 12299
312 10809
314 9883
316 9290
318 8830
320 8508
322 8255
324 8046
326 7875
328 7639
330 7204
332 6607
334 5850
336 5031
338 4262
340 3621
342 3058
344 2641
346 2336
348 2081
350 1886
352 1752
354 1590
356 1456
358 1346
360 1263
362 1187
364 1210
366 1128
368 1065
370 1067
372 1070
374 1071
376 1091
378 1117
380 1099
382 1141
384 1155
386 1177
388 1248
390 1257
392 1294
394 1283
396 1271
398 1248
400 1143
402 1085
404 990
406 899
408 777
410 693
412 687
414 616
416 575
418 543
420 598
422 629
424 565
426 589
428 662
430 711
432 652
434 769
436 783
438 861
440 900
442 943
444 1010
446 1041
448 1090
450 1169
452 1253
454 1303
456 1405
458 1556
460 1500
462 1637
464 1791
466 1735
468 1854
470 1825
472 2037
474 2022
476 2173
478 2254
480 2324
482 2429
484 2631
486 3055
488 2548
490 2773
492 2744
494 2730
496 2911
498 2902
500 2847
502 2927
504 2858
506 2889
508 2911
510 2919
512 2892
514 2916
516 2901
518 2977
520 2965
522 2998
524 3142
526 3319
528 3319
530 3461
532 3677
534 3832
536 3951
538 4344
540 4468
542 4833
544 5093
546 5409
548 5843
550 6189
552 6720
554 7094
556 7613
558 8080
560 8724
562 9381
564 10172
566 10892
568 11499
570 11981
572 12527
574 13318
576 14130
578 14954
580 16093
582 16927
584 18402
586 19208
588 20767
590 21983
592 23962
594 25365
596 26811
598 28807
600 30580
602 32239
604 33577
606 35201
608 36035
610 37418
612 38121
614 38626
616 38847
618 39148
620 39384
622 39694
624 39847
626 40321
628 40968
630 41725
632 42960
634 44176
636 46129
638 47803
640 49754
642 51895
644 54622
646 57263
648 60272
650 62654
652 66008
654 68935
656 73230
658 70273
660 71547
662 73004
664 74028
666 74014
668 73044
670 71089
672 67725
674 63368
676 57423
678 50928
680 44792
682 38591
684 31941
686 27209
688 22710
690 18448
692 14973
694 12227
696 9865
698 7947
700 6511
702 5331
704 4303
706 3670
708 2866
710 2406
712 2103
714 1750
716 1389
718 1297
720 1028
722 926
724 948
726 868
728 620
730 702
732 526
734 607
736 519
738 294
740 432
742 233
744 298
746 365
748 433
750 394
752 217
754 391
756 296
758 331
760 154
762 73
764 278
766 -2
768 246
770 253
772 -50
774 5
776 -2
778 221
780 311
782 63
784 -23
786 -6
788 156
790 93
792 40
794 223
796 -73
798 50
800 121